Increased activities of hepatic orotidine 5′-phosphate pyrophosphorylase and orotidine 5′-phosphate decarboxylase induced by orotate
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منابع مشابه
Orotidine-5'-phosphate decarboxylase and pyrophosphorylase of bean leaves.
This report includes results demonstrating the existence of orotidine-5'-phosphate decarboxylase and orotidine-5'-phosphate pyrophosphorylase in plant leaves. The decarboxylase enzyme, purified 8 fold from leaves of etiolated pinto beans (Phaseolus vulgaris L.), had a pH optimum of 6.3. It was strongly inhibited by 6-azauridine-5'-phosphate; a concentration of 12 mum decreased the reaction rate...
متن کاملTransformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.
Relief of an auxotrophic requirement for uridine in Aspergillus nidulans strain G191 has been achieved by transformation with a segment of Neurospora crassa DNA containing the corresponding gene coding for orotidine-5'-phosphate decarboxylase. The mitotic stability of such transformants suggests that the DNA has integrated into the genome. Southern hybridisation analysis of DNA isolated from tr...
متن کاملThe crystal structure and mechanism of orotidine 5'-monophosphate decarboxylase.
The crystal structure of Bacillus subtilis orotidine 5'-monophosphate (OMP) decarboxylase with bound uridine 5'-monophosphate has been determined by multiple wavelength anomalous diffraction phasing techniques and refined to an R-factor of 19.3% at 2.4 A resolution. OMP decarboxylase is a dimer of two identical subunits. Each monomer consists of a triosephosphate isomerase barrel and contains a...
متن کاملKinetic properties and inhibition of orotidine 5'-phosphate decarboxylase. Effects of some allopurinol metabolites on the enzyme.
The land 7-ribosyl 5’-phosphates of oxipurinol [4,6dihydroxypyrazolo(3,4 d)pyrimidine] were enzymatically synthesized. These nucleotides, 3-xanthosine S’-phosphate, and the 1-ribosyl 5’-phosphate of allopurinol [4-hydroxypyrazolo(3,4-d)pyrimidine] were characterized by spectral, chromatographic, and enzymatic procedures. The oxipurinol nucleotides were found to be potent competitive inhibitors ...
متن کاملRate and Equilibrium Constants for an Enzyme Conformational Change during Catalysis by Orotidine 5′-Monophosphate Decarboxylase
The caged complex between orotidine 5'-monophosphate decarboxylase (ScOMPDC) and 5-fluoroorotidine 5'-monophosphate (FOMP) undergoes decarboxylation ∼300 times faster than the caged complex between ScOMPDC and the physiological substrate, orotidine 5'-monophosphate (OMP). Consequently, the enzyme conformational changes required to lock FOMP at a protein cage and release product 5-fluorouridine ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1972
ISSN: 0014-5793
DOI: 10.1016/0014-5793(72)80099-1